HUMA QURESHI
About Candidate
Location
Education
Doctor of Philosophy thesis on “tat gene of HIV-1 Indian subtype C as a vaccine candidate for immunogenicity testing in Balb/C mice”. Chief adviser: Dr. Pradeep Seth
tat gene of HIV-1 Indian subtype C was amplified and sequenced. Subtype C tat consensus was derived, mutated and codon optimized according to human codon frequency and cloned in m a m m a l i a n expression vector (pNK14) and MVA vector to construct rDNA/rMVA vaccine. Immunogenicity of tat rDNA/MVA vaccine was evaluated in mouse
Work & Experience
Evaluation of an immune responses induced by Oral Ad5 EV68 vaccine in mice.
Understanding of immune responses induced by multi-antigenic EBV vaccine in Rabbits.
Design and development of Pan SARSCOV2 mRNA-based nanoparticle vaccine.
Preclinical testing of Pan SARSCOV2 mRNA-based nanoparticle vaccine in animal models and immunized human subjects.
SARSCOV2 specific immunoassay development and validation.
• Screening of a cohort of HIV-1 positive patients to detect neutralizing antibodies.
• Blood sample processing to separate PBMCs and serum for immunoassays.
• Monoclonal Antibody isolation using Flowcytometry based method
(Single cell method).
• Characterization of engineered Monoclonal antibodies (Half-life variants).
• High Throughput Assays (Cell based; Neutralization assay /Serum based; ELISA)
• Natural immunity and vaccine induced immunity.
• PK/PD studies of engineered Half-life variants of monoclonal antibodies in mice.
• Development of B cell Activation assay to screen and select HIV immunogen for preclinical and clinical studies.
• Isolation of broadly neutralizing antibodies from PBMC’s of HIV infected patients.
• Understand the role of cytoplasmic tail of HIV envelope protein in maintaining trimeric native like conformation.
• Teaching immunology course to graduate students.
• Teaching and training technicians and junior staff.
Development of penile transmission model of SIV in adult male rhesus macaques.
Immunogenicity testing of Merck Ad5SIV/HIV vaccine in adult male rhesus macaque model.
Role of pre-existing Ad5 immunity in increasing susceptibility to SIV/HIV infection.
Virology and immunology of adenovirus infection in rhesus macaques.
•Modulation of innate and adaptive immunity of rhesus monkey after Ad5 infection.
Development of Treg suppression assay for preclinical vaccine studies in rhesus macaques.
Evaluation of HSV-1 specific immune responses elicited by HSV-1 asymptomatic and symptomatic human subjects.
Training and mentoring undergraduate students in virology and immunology lab techniques.
